| 湖南省牛无浆体虫株分子生物学鉴定 |
| |
| 引用本文： | 何德肆,李海辉,欧阳叙向,张明,胡述光,刘毅.湖南省牛无浆体虫株分子生物学鉴定[J].动物医学进展,2009,30(12):43-47. |
| |
| 作者姓名： | 何德肆 李海辉 欧阳叙向 张明 胡述光 刘毅 |
| |
| 作者单位： | 1. 湖南生物机电职业技术学院,湖南长沙,410127
2. 湖南农业大学动物医学院,湖南长沙,410128 |
| |
| 摘 要： | 用原虫16 S rRNA基因序列通用引物对湖南5个地区牛无浆体感染的阳性血液样品进行PCR扩增,经测序和序列拼接,获得5个无浆体样品16 S rRNA基因的部分序列,应用分子生物学软件进行分析,并根据所获得序列的保守区间设计特异性引物,进行PCR诊断方法的探讨性研究.结果表明,在通用引物下,5个地区无浆体感染的阳性血液样品均获得1 425 bp大小的虫体16 S rRNA基因条带;测序后5个无浆体16 S rRNA序列同源性均在97%～98%.证明5个分离虫株初步鉴定为牛边缘无浆体.
|
| 关 键 词： | 牛 无浆体病 分子生物学 鉴定 |
Study on Molecular Biology Identification of Bovine Anaplasma Isolates in Hunan |
| |
| HE De-si,LI Hai-hui,OUYANG Xu-xiang,ZHANG Ming,HU Shu-guang,LIU Yi.Study on Molecular Biology Identification of Bovine Anaplasma Isolates in Hunan[J].Progress In Veterinary Medicine,2009,30(12):43-47. |
| |
| Authors: | HE De-si LI Hai-hui OUYANG Xu-xiang ZHANG Ming HU Shu-guang LIU Yi |
| |
| Institution: | HE De-si1,LI Hai-hui2,OUYANG Xu-xiang1,ZHANG Ming2,HU Shu-guang1,LIU Yi2 (1.Hunan Biological And Electromechanical College,Changsha,Hunan,410127,China,2.College of Veterinary Medicine,Hunan Agricultural University,410128,Chin) |
| |
| Abstract: | The 16 S RNA gene sequence of Anaplasma was amplificated from positive blood in cattle with Anaplasma infection in five different districts of Hunan with the universal primers of protozoa.PCR products were partially sequenced and spliced,and 16sRNA gene partial sequence of Anaplasma isolated from different districts in Hunan were analyzed or identified via molecular biological software. At last, a specific PCR diagnosis method on Anaplasma was researched with a pair of specific designed primers in the conse... |
| |
| Keywords: | cattle anaplasmosis molecular biology identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
