A high-throughput method for early screening of coffee (<Emphasis Type="Italic">Coffea</Emphasis> spp.) genotypes for resistance to root-knot nematodes (<Emphasis Type="Italic">Meloidogyne</Emphasis> spp.) |
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Authors: | Luc Villain Jamel Aribi Georges Réversat François Anthony |
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Institution: | (1) IRD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5, France;(2) CIRAD, UMR RPB, BP 64501, 34032 Montpellier Cedex 5, France; |
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Abstract: | Root-knot nematodes (Meloidogyne spp.) threaten the livelihood of millions of farmers producing coffee worldwide. The use of resistant plants either as cultivars
or rootstocks appears to be the single most effective method of control. A screening method was developed to evaluate large
populations of plants for resistance to root-knot nematodes. Two coffee cultivars, one susceptible and the other resistant
to Meloidogyne paranaensis, were grown under controlled conditions in two substrates: a commercial sieved potting compost and an inert substrate containing
sand with a water-absorbent synthetic polymer. Plant growth and development and nematode multiplication were compared for
two inoculation dates (2 and 8 weeks after planting) and two evaluation dates (eight and 13 weeks after inoculation). Root
growth, but not nematode multiplication, was influenced by the choice of substrate. Evaluation of the differences in root
weight and nematode numbers between the different cultivars, substrates and dates of inoculation suggested that an optimal
condition could be defined. The best discrimination between susceptible and resistant plants was found in the experiment where
inoculation occurred at 2 weeks after planting and evaluation occurred at 8 weeks after inoculation. Because the total duration
of this experiment was only 3 months, high-throughput evaluation was possible, opening up new possibilities for screening
large germplasm collections and studying the genetic control of root-knot nematode resistance in coffee. |
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