| 五唇兰基因组DNA提取方法的优化 |
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| 引用本文： | 司更花,朱国鹏.五唇兰基因组DNA提取方法的优化[J].热带林业,2012(2):37-39. |
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| 作者姓名： | 司更花 朱国鹏 |
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| 作者单位： | 海南大学热带作物种质资源保护与开发利用教育部重点实验室;海南大学园艺园林学院 |
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| 基金项目： | 海南省科技成果示范推广专项项目(CGSF20120001-02);林业公益性行业科研专项(201204604) |
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| 摘 要： | 五唇兰组织内含有大量的酚类、多糖以及此生代谢产物,DNA提取较为困难。为获得高质量的五唇兰基因组DNA,文中采用传统十六烷基三甲基溴化铵(CTAB)法、常规十二烷基硫酸钠(SDS)法和改进的CTAB法对五唇兰基因组DNA的提取进行比较。结果表明:改进的CTAB法比传统CTAB法和常规SDS法都好,可以得到质量较好的DNA,电泳条带清晰,通过紫外分光光度计检测,A260/A280为2.0以上。
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| 关 键 词： | 五唇兰 基因组DNA 提取 |
Optimization of Extraction of Genomic DNA in Phalaenopsis pulcherrima(Orchidaceae) |
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| Si Genghua,Zhu Guopeng.Optimization of Extraction of Genomic DNA in Phalaenopsis pulcherrima(Orchidaceae)[J].Tropical Forestry,2012(2):37-39. |
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| Authors: | Si Genghua Zhu Guopeng |
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| Institution: | 1.Key Laboratory of Protection and Developmental Utilization of Tropical Crop Germplasm Resources(Hainan University),Ministry of Education,Haikou,Hainan570228,China; 2.College of Horticulture and Landscape Architecture,Hainan University,Haikou,,Hainan 570228,China) |
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| Abstract: | Phalaenopsis pulcherrima is rich in polysaccharides,phenol and other secondary metabolites,and it is difficult to obtain high quality of genomic DNA from it.Based on the research results of comparative analysis of several extraction methods,such as traditional Hexadecyltrimethy Ammonium Bromide(CTAB),traditional Sodium Dodecyl Sulfate(SDS) and improved CTAB,for total genomic DNA of P.pulcherrima.The results indicate that improved CTAB DNA extraction produces DNA of much better quality than the others,which has higher density,purity and intact genomic DNA by agarose gel electrophoresis.And by ultraviolet spectrophometry,the ratio of A260/A280 was above 2.0. |
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| Keywords: | Phalaenopsis pulcherrima Genomic DNA Extraction |
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