| 东方蜜蜂DNA随机扩增多态及其遗传分化研究 |
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| 引用本文： | 董霞,张炫,史宪伟,汪建明,陈永久,张亚平. 东方蜜蜂DNA随机扩增多态及其遗传分化研究[J]. 中国蜂业, 2002, 53(2): 9-10,18 |
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| 作者姓名： | 董霞 张炫 史宪伟 汪建明 陈永久 张亚平 |
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| 作者单位： | [1]云南农业大学东方蜜蜂研究所，昆明650201 [2]中国科学院昆明动物所细胞与分子进化开放实验室，昆明650223 |
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| 基金项目： | 云南省自然科学基金资助项目 (项目编号 :97C0 0 6Q) |
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| 摘 要： | 本研究对云南及马来西亚的37个东方蜜蜂样本进行随机增多态DNA分析，从20个引物中筛选出11个引物，其中9个引物扩增出多态带。共检测到66条扩增片段，其中56条为多态带。用UPGMA聚类方法构建的分子系统树显示，云南的样本、马来西亚的样本各自分别聚在一起，说明两个样本间遗传差异较大，群体之间存在着遗传分化。但就云南的32个个体而言，虽然聚类图中大多数采用自同一地区的样本聚在一起，但也存在一定交叉，提示云南地理群体间近期可能存在一定的基因流。
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| 关 键 词： | 东方蜜蜂 RAPD 遗传分化 DNA随机扩增多态 |
| 文章编号： | 0412-4367(2002)02-0009-03 |
Genetic divergences of apis cerana from Yunnan of China and Malaysia were estimated by rapd analysis |
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| Dong Xia+ Zhang Xian+ Shi Xianwei+ Wang Jianming+ Chen Yongjiu+ Zhang Yaping+. Genetic divergences of apis cerana from Yunnan of China and Malaysia were estimated by rapd analysis[J]. Apiculture of China, 2002, 53(2): 9-10,18 |
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| Authors: | Dong Xia+ Zhang Xian+ Shi Xianwei+ Wang Jianming+ Chen Yongjiu+ Zhang Yaping+ |
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| Affiliation: | Dong Xia+1 Zhang Xian+1 Shi Xianwei+2 Wang Jianming+1 Chen Yongjiu+2 Zhang Yaping+2 |
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| Abstract: | In this study,random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 37 Apis cerana workers individuals from Yunnan of China and from Malaysia.20 primers were used among which 9 generated polymorphic loci.A total of 66 bands were scored,of which 56(84.85%)were polymorphism.The genetic distance matrix was obtained by proportion of shared fragments.Dendrogram was generated by UPGMA.There were genetic divergences among Apis cerana from Yunnan province of China and from Malaysia.; |
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| Keywords: | Apis cerana Yunnan Malaysia RAPD Genetic divergence |
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