celB基因标记法研究酸性土花生接种及施钼效果

以缺钼酸性紫色土为供试土壤做盆栽试验,选用celB标记的三株慢生型花生根瘤菌celB3-5、celB3-7、celB4-5接种天府9号花生。通过标记根瘤菌形成的根瘤能与检测试剂产生颜色反应的特征,检测施钼及施不同浓度的钼对花生-根瘤菌有效性和竞争性的影响。结果表明,缺钼酸性紫色土上单施钼、单接种、接种配合施钼均能促进花生与根瘤菌的共生固氮效应和竞争结瘤能力,但接种配合施钼的效果最好,单接种的效果次之,单施钼的效果差。单施钼时,0.4%的钼酸铵拌种效果好,接种根瘤菌时,0.2%的效果好。供试菌株中celB4-5的有效性和竞争性最强,celB3-7次之,celB3-5差。

Study the effects of Mo and inoculation of Bradyrhizobium in acid purple soil by celB gene maker

In this research,an acid purple soil deficient in Mo was used for pot experiment.Three strains of celB-labeled Bradyrhizobium celB3-5、celB3-7、celB4-5 were selected to inoculate Tianfu 9 peanut.Root nodule formed by gene marker strains would have color reaction with the test reagent.This characteristic can be used to detect effect of Mo and its application rate on the effectiveness and competitiveness of the peanut-Bradyrhizobium symbiosis.Results show that in the acid purple soil Mo deficit,single application of Mo,single inoculation of Bradyrhizobium or combination of the two all could promote symbiotic nitrogen-fixation and competitive ability of peanut-Bradyrhizobium in nodulation,(among) which the effect of combination was the best,followed by inoculation and single Mo application.The effect of application of 0.4% NH₄MoO₃ solution was more pronounced than that of 0.2% NH₄MoO₃ solution when applied alone,whereas the effect of the former was less pronounced than that of the latter when combined with inoculation.In terms of effectiveness and competitiveness,Strain celB4-5 took the best and followed with celB37 and celB3-5.