| 标记基因nptⅡ在转基因苹果嫁接砧穗间无相互传导 |
| |
| 引用本文： | 周瑞金,杜国强,师校欣.标记基因nptⅡ在转基因苹果嫁接砧穗间无相互传导[J].园艺学报,2006,33(6):1329-1330. |
| |
| 作者姓名： | 周瑞金 杜国强 师校欣 |
| |
| 作者单位： | (河北农业大学园艺学院, 河北保定071001) |
| |
| 基金项目： | 河北省自然科学基金资助项目(C2004000368) |
| |
| 摘 要： | 以携带外源新霉素磷酸转移酶基因( nptⅡ基因) 苹果品种嘎拉、未进行基因转化的普通型嘎拉和苹果砧木M26组培苗为试材, 通过试管微嫁接方法, 对其体内所含外源nptⅡ基因在砧穗中的传导性进行了研究。结果表明, 外源nptⅡ基因只在转化植株体内表达, 不通过嫁接在接穗和砧木间进行传导。
|
| 关 键 词： | 苹果 转基因 微嫁接 传导 |
| 文章编号： | 0513-353X（2006）06-1329-02 |
| 收稿时间： | 2006-08-01 |
| 修稿时间： | 2006-08-012006-11-21 |
Non-conducting of Report Gene in Transgenic Apple by Micro-grafting in Vitro |
| |
| Zhou Ruijin,Du Guoqiang,Shi Xiaoxin.Non-conducting of Report Gene in Transgenic Apple by Micro-grafting in Vitro[J].Acta Horticulturae Sinica,2006,33(6):1329-1330. |
| |
| Authors: | Zhou Ruijin Du Guoqiang Shi Xiaoxin |
| |
| Institution: | (College of Horticulture, Hebei Agricultural University, Baoding, Hebei 071001, China) |
| |
| Abstract: | The conducting effect of the report gene ( Neomycin phosphotransferaseⅡ, nptⅡ) between the rootstocks and scions was studied by using micro-grafting of the transgenic lines of Malus domestica‘Gala’, wild type Gala, and apple rootstock of M26 in vitro. The results showed that the exogenous nptⅡ genewas only expressed in transgenic explants and it did not conduct between the scions and rootstocks by micrografting. |
| |
| Keywords: | Apple Transgenic Micro-grafting Conduction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
