九种李属植物的RAPD亲缘关系分析

 以李属植物9 个种的20 个材料为研究对象, 用RAPD技术对其进行亲缘关系分析。在建立适合李属植物的PCR2RAPD反应体系的基础上, 从45 个随机引物(10 mer) 中筛选出24 个, 对所有供试材料进行扩增, 共获得24 张DNA 指纹图谱, 326 条DNA 谱带, 其中有311 条为多态带。建立了基于RAPD 的李属植物亲缘关系树形图, 树形图的聚类结果与经典的李属植物的起源、分布和分类基本一致。另外, 根据聚类结果, 作者认为: 1) 乌苏里李是中国李的一个变种而非一独立的种; 2) 杏李是一李杏杂种且与杏有较近的亲缘关系; 3) 从分子水平证明了欧洲李(Prunus domestica L. ) 是由樱桃李(P. cerasifera Ehrh. ) 和黑刺李(P. spinosa L. ) 自然杂交形成的后代。

Phylogenetic Relationship among Nine Prunus Species Based on Random Ampli-fied Polymorphic DNA

In this study, nine species of Prunus (20 materials)were analyzed using random amplified polymorphic DNA markers. 24 primers were screened from 45 arbitrary 10 mer primers, and a total of 326 DNA bands were amplified, 311 of which (95.4%) were polymorphism. According to the data,a tree diagram of phylogenetic relationship was constructed using UPGMA in NYSYS. The results showed that: 1) P. ussuriensis was a variety of P. salician , not a independent species; 2) P. simonii was a hybrid of P. salician and P. armaniaca and was closely related to P. armaniaca ; 3) P. domestica was a offspring formed by naturally crossing between P. cerasifera and P. spinosa .