| 磺胺对甲氧嘧啶全抗原的制备研究 |
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| 引用本文： | 周兰香,徐忠民,李幼荣,王宗元. 磺胺对甲氧嘧啶全抗原的制备研究[J]. 扬州大学学报(农业与生命科学版), 2001, 22(4): 55-58 |
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| 作者姓名： | 周兰香 徐忠民 李幼荣 王宗元 |
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| 作者单位： | 1. 扬州大学理学院化学化工系,
2. 扬州大学畜牧兽医学院动物医学系, |
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| 基金项目： | 江苏省自然科学基金资助项目 (BK990 93 ) |
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| 摘 要： | 采用戊二醛法 ,将半抗原磺胺对甲氧嘧啶 (SMD)与载体牛血清白蛋白 (BSA)偶联 ,制得磺胺对甲氧嘧啶全抗原 SMD-BSA。其紫外扫描光谱和红外光谱表明偶联成功。通过改变半抗原 SMD和偶联剂戊二醛的用量 ,制得多种结合比的全抗原 ,这为进一步制备抗 SMD抗体提供了良好的免疫原
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| 关 键 词： | 磺胺对甲氧嘧啶 全抗原 牛血清白蛋白 紫外扫描光谱 红外光谱 |
| 文章编号： | 1000-2049(2001)-04-0055-04 |
| 修稿时间： | 2001-09-01 |
STUDY ON PREPARATION OF COMPLETE ANTIGEN OF SULFAMETHOXYDIAZINE |
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| ZHOU Lan xiang ,XU Zhong min ,LI You rong ,WANG Zong yuan. STUDY ON PREPARATION OF COMPLETE ANTIGEN OF SULFAMETHOXYDIAZINE[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2001, 22(4): 55-58 |
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| Authors: | ZHOU Lan xiang XU Zhong min LI You rong WANG Zong yuan |
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| Affiliation: | ZHOU Lan xiang 1,XU Zhong min 1,LI You rong 1,WANG Zong yuan 2 |
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| Abstract: | Semiantigen sulfamethoxydiazine (SMD) was coupled with carrier protein bovine serum albumin (BSA) to prepare complete antigen SMD BSA by glutaraldehyde method. Both UV scanning spectrum and IR spectrum coupling occurred between SMD and BSA. A series of SMD BSA with different combination ratios was synthesized through altering the use level of semiantigen SMD or the use level of coupling agent glutaraldehyde. The present research supplied an excellent immune antigen for further preparation of antibody to SMD. |
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| Keywords: | sulfamethoxydiazine complete antigen bovine serum albumin UV scanning spectrum IR spectrum |
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