Basic biological characterization of feline morbillivirus |
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Authors: | Rie KOIDE Shoichi SAKAGUCHI Takayuki MIYAZAWA |
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Institution: | 1)Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606–8507, Japan |
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Abstract: | Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered
in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic
nephritis, little is known about its biological characteristics. In this study, we
established a quantitative assay of FmoPV by using an indirect immunofluorescence
technique. Viral titers of FmoPV were determined in one week. Treatment with
polybrene® or trypsin which was previously used in virus isolation did not
augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at
least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2
min, respectively. The biological characteristics of FmoPV reported here will be
beneficial for establishing an efficient virus isolation method and will provide important
information to take a measure to reduce the risk of FmoPV infection. |
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Keywords: | feline feline morbillivirus quantification replication kinetics stability |
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