The genetic organization of the capsular polysaccharide biosynthesis region
of Actinobacillus pleuropneumoniae serotype 14 |
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| Authors: | Hiroya ITO |
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| Affiliation: | 1)National Institute of Animal Health, National Agriculture and Food Research Organization, 3–1–5 Kannondai, Tsukuba, Ibaraki, Japan |
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| Abstract: | The genetic organization of the gene involved in the capsular polysaccharide(CPS) biosynthesis of Actinobacillus pleuropneumoniae serotype 14 hasbeen determined. The DNA region for the CPS biosynthesis of serotype 14(cps14) comprised 9 open reading frames, designated ascps14AB1B2B3CDEFG genes, encodingCps14A to Cps14G protein, respectively. Cps14A was similar to CpsA of A.pleuropneumoniae serotypes 1, 4 and 12; the Cps14B1 andCps14B2 were similar to CpsB of A. pleuropneumoniaeserotypes 1, 4 and 12, suggesting that CPS structure of A.pleuropneumoniae serotype 14 would belong to Group I including A.pleuropneumoniae serotypes 1, 4, 12 and 15. Surprisingly, the overallnucleotide sequence, deduced amino acid sequence, and the genetic organization of thecps14 were nearly identical to those of Actinobacillussuis. This study will provide the molecular basic knowledge for development ofdiagnostics and vaccine of A. pleuropneumoniae serotype 14. |
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| Keywords: | Actinobacillus pleuropneumoniae serotype 14 Actinobacillus suis capsular polysaccharide biosynthesis genetic organization |
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