固相抗体直接竞争EL ISA 法测定氯黄隆在土壤中的残留动态

采用包被抗体直接竞争EL ISA 法测定了氯黄隆在盆土中的残留动态和农田土壤中氯黄隆的残留量。结果表明: EL ISA 法测定土壤中氯黄隆的检测限2、45 g/ hm2 第一年麦收时中性沙质土壤和弱碱性沙壤土中氯黄隆的残留量分别为0. 24、0. 56 ng/g 和0. 30、0. 96 ng/g, 第二年分别为0. 28、0. 67 ng/g 和0. 38、1. 09 ng/g , 与平行进行的高效液相色谱法、生物测定法测定结果基本一致。

Residue Dynamics of Chlorsulfuron in Soil Determined byAntibody Immobilized Direct Competitive ELISA

A highly specific and sensitive direct competitive (immobilized antibody, enzyme tagged hapten) ELISA was developed to rapidly monitor the residue dynamics of chlorsulfuron in potted soil and cropland soil. The limit of detection was lower than 0.1 ng/g, the recoveries were 85.4%~118%, the CVs(%) were 8.95%~16.14% and the half lives were 22, 26 days for chlorsulfuron in potted soil at 10, 1000 ng/g spiking levels. The residues, at the time of wheat harvest, were 0.24 ng/g and 0.56 ng/g in sandy soil from nonirrigated cropland, 0.30 ng/g and 0.96 ng/g in sandy loam from wheat rice land for the first year, 0.28 ng/g and 0.67 ng/g, 0.38 ng/g and 1.09 ng/g for second year when 22.5g /hm 2 and 45g /hm 2 of chlorsulfuron had been used to control wheat land weed for two years. The results from ELISA were well correspondent with that from HPLC and bioassay.