Proteomic analysis of porcine saliva |
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| Authors: | AM Gutirrez I Miller K Hummel K Nbauer S Martínez-Subiela E Razzazi-Fazeli M Gemeiner JJ Cern |
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| Institution: | a Department of Animal Medicine and Surgery, University of Murcia, 30100 Espinardo, Murcia, Spain;b Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria;c VetOMICS Core Facility for Research, University of Veterinary Medicine Vienna, Veterinaerplatz 1, A-1210 Vienna, Austria |
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| Abstract: | Saliva contains a number of proteins that may be useful as biomarkers of health and disease and can be easily obtained from large numbers of animals in a non-invasive, stress-free way. The objective of this study was to explore the protein composition of porcine saliva from 10 specific pathogen free pigs using first one-dimensional SDS–PAGE and then two-dimensional electrophoresis and mass spectrometry. A reference proteome pattern for porcine saliva was established with the identification of 13 different, mainly saliva-specific, proteins. These reference data will facilitate the investigation of salivary proteins potentially altered in disease and could serve as novel diagnostic biomarkers. |
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| Keywords: | Proteome Saliva Porcine Two-dimensional electrophoresis Mass spectrometry Immunoblotting Biomarkers |
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