| 茶尺蠖普通气味结合蛋白(GOBP)的基因克隆与序列分析 |
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| 引用本文： | 陈华才,刘军,张晓燕,殷坤山.茶尺蠖普通气味结合蛋白(GOBP)的基因克隆与序列分析[J].茶叶科学,2010,30(1). |
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| 作者姓名： | 陈华才 刘军 张晓燕 殷坤山 |
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| 作者单位： | 1. 中国计量学院,浙江,杭州,310018
2. 中国农业科学院茶叶研究所,浙江,杭州,310008 |
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| 基金项目： | 浙江省自然科学基金(Y305130) |
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| 摘 要： | 以触角总RNA为模板,根据同源性设计简并性引物,采用RT-PCR扩增结合RACE技术克隆获得了茶尺蠖(Ectropis obliqua)普通气味结合蛋白GOBP1和GOBP2基因的cDNA全长序列。分别命名为EoblGOBP1和EoblGOBP2。EoblGOBP1的cDNA全长为1 528 bp,开放阅读框501 bp,编码166个氨基酸残基,预测分子量Mw=18 835.63 D,等电点pI=5.45,cDNA和氨基酸序列在NCBI/GenBank的登录号分别为FJ156732和ACN29680.1。EoblGOBP2的cDNA全长为1 315 bp,开放阅读框405 bp,编码160个氨基酸残基,预测分子量Mw=18 002.75 D,等电点pI=5.32。cDNA和氨基酸序列在NCBI/GenBank的登录号分别为FJ156733和ACN29681.1。两个气味结合蛋白的氨基酸序列中都含有6个保守的半胱氨酸位点,具有气味结合蛋白的典型特征,与已报道的鳞翅目昆虫气味结合蛋白的同源性为分别62%～82%和76%～88%。
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| 关 键 词： | 茶尺蠖 普通气味结合蛋白(GOBP) 全长序列 基因克隆 序列分析 |
Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina |
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| CHEN Hua-cai,LIU Jun,ZHANG Xiao-yan,YIN Kun-shan.Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina[J].Journal of Tea Science,2010,30(1). |
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| Authors: | CHEN Hua-cai LIU Jun ZHANG Xiao-yan YIN Kun-shan |
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| Institution: | 1.China Jiliang University;Hangzhou 310018;China;2.Tea Research Institute of Chinese Academy of Agricultural Sciences;Hangzhou 310008;China |
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| Abstract: | The full-length sequences of two general odorant binding protein(GOBP) genes in Ectropis obliqua(named EoblGOBP1 and EoblGOBP2) were cloned with degenerated digonudeotide primers by RT-PCR and the rapid amplification of cDNA ends(RACE) using the total RNA isolated from the antenna of Ectropis obliqua as template.The length of EoblGOBP1 was 1 528 bp with a 501bp ORF which encoded a peptide of 166 amino acid residues.The predicted Mw and pI of the peptide were 18 835.63 D and 5.45 respectively.The NCBI/GenBan... |
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| Keywords: | Ectropis oblique general odorant binding protein full-length sequence gene cloning sequence analysis |
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