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蔗糖与麦芽糖的配比对小麦花粉愈伤组织诱导的分化频率的影响
引用本文:陈耀锋 朱庆麟. 蔗糖与麦芽糖的配比对小麦花粉愈伤组织诱导的分化频率的影响[J]. 作物学报, 1993, 19(2): 145-148
作者姓名:陈耀锋 朱庆麟
作者单位:西北农业大学农学系,陕西,杨陵,712100
摘    要:通过小麦花药培养基中蔗糖(Su)与麦芽糖(Ma)的配合使用研究,发现蔗糖与麦芽糖在最适配比时,能显著提高小麦花药培养效率.含9%蔗糖和2%麦芽糖的诱导培养基,花粉愈伤组织诱导频率和绿苗产量比仅含11%蔗糖的对照有显著的提高.诱导、分化培养基中同时使用适当配比的芳糖和麦芽糖,小麦花药培养效率提高更显著,在最佳组合时,绿苗

关 键 词:小麦,花药培养,效率,绿苗产量
收稿时间:1990-12-30
修稿时间:1992-08-14

Effect of the Ratio of Sucrose to Maltose on the Frequency of Induction and Differentiation of Pollen Callus in Wheat
Chen Yao-feng,Zhu Qing-lin Agronomy Department,Northwestern Agricultural University,Yangling,Shaanxi. Effect of the Ratio of Sucrose to Maltose on the Frequency of Induction and Differentiation of Pollen Callus in Wheat[J]. Acta Agronomica Sinica, 1993, 19(2): 145-148
Authors:Chen Yao-feng  Zhu Qing-lin Agronomy Department  Northwestern Agricultural University  Yangling  Shaanxi
Affiliation:Agronomy Department,Northwestern Agricultural University,Yangling,Shaanxi,712100
Abstract:Efficiency of wheat auther culture was studied using media containing sucrose andmaltose. It was found that the optimum ratio of sucrose and maltose in the inductionmedium could increase the efficiency of wheat anther culture significantly. When 9%sucrose and 2% maltose were added to the induction medium, the frequency of pollencalli induced and the % of green plantlets were significantly higher than those of thecontrol which cantained 11% sucrose only. Using the most suitable ratio of sucroseand maltose in both media could increase the efficiency of anther culture even better.It was also found that the reduction of sucrose and the increase of maltose could leadto higher % of green plantlets and G/A ratio.
Keywords:Wheat  Anther culture  Efficiency  Green plantlet%  
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