| 正交设计龙胆提取工艺的研究 |
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| 引用本文： | 王艳艳,王英平. 正交设计龙胆提取工艺的研究[J]. 特产研究, 2007, 29(2): 15-17 |
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| 作者姓名： | 王艳艳 王英平 |
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| 作者单位： | 中国农业科学院特产研究所,吉林,吉林,132109 |
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| 基金项目： | 科技部人参等中药提取生物技术研究项目;吉林省科学技术厅中药现代化项目 |
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| 摘 要： | 目的建立龙胆最佳提取工艺,为龙胆临床及药理研究奠定基础。方法试验采用超声提取法,以溶剂浓度(A)、超声时间(B)和料液比(C)为三因素,设计正交试验,优化龙胆提取工艺;同时,应用HPLC测定各组合龙胆苦苷含量。结果影响因素最大的是溶剂浓度,其次是超声时间,影响最小的是料液比。结论各因素较优的水平组合为A2B3C1,即用乙醇浓度为50%、超声时间为70min、料液比为1:4的条件下,提取龙胆得膏率最高。
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| 关 键 词： | 龙胆 正交设计 提取 |
| 文章编号： | 1001-4721（2007）02-0015-03 |
| 修稿时间： | 2007-02-02 |
Research for the Best Extracting-craftwork about Available Component of Gentiana scabra Bunge |
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| WANG Yan-yan,WANG Ying-ping. Research for the Best Extracting-craftwork about Available Component of Gentiana scabra Bunge[J]. Special Wild Economic Animal and Plant Research, 2007, 29(2): 15-17 |
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| Authors: | WANG Yan-yan WANG Ying-ping |
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| Affiliation: | Institute of Special Wild Economic Animal and Plant Science, CAAS.Jilin 132109, China |
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| Abstract: | Objectivev For further researching on the clinic and pharmacology action of G.scabra Bge,it Was founded for the best extracting craftwork of G.scabra Bunge.Methods the study applied on super-sonic extracting method.we designed orthogonal experiment by three facts for example solvent concentration(A),super-sonic time(B),liquid-material ratio(C).Gentiopicrin content was detected with HPLC.Result the solvent concentration(A) is the most effective factor.the second is super-sonic time(B).the last is liquid-material ratio(C).Conclution the best extracting craftwork is A2B3C1 : ethanol concentration50%,super-sonic time70min,material-liquid ratio1:4. |
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| Keywords: | Gentiana scabra Bunge Designed orthogonal Extracting |
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