An immunoenzyme test used for the detection of antibodies to rinderpest: the advantage of using a purified virus cultivated in a cell line |
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| Authors: | G Libeau D Calvez |
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| Affiliation: | Institut d'Elevage et de Médecine Vétérinaire des Pays Tropicaux/CIRAD, Service de Pathologie Infectieuse, Maisons-Alfort, France. |
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| Abstract: | Two types of in vitro assays (enzyme immunoassay and sero-neutralization test) were compared for their ability to detect antibodies to rinderpest virus in field sera from West African bovines. Purified virus grown on Vero cells (Ag/Vero) or bovine kidney cells (Ag/BK), were tested as antigen in enzyme-linked immunoassays (EIA). The results of the comparative evaluation of the two antigens by EIA, prove that Ag/Vero did enhance the sensitivity and the specificity of the test in comparison to Ag/BK and offers a 94% correspondence with seroneutralization. |
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