| 猪链球菌通用及2型TaqMan荧光PCR检测技术建立与应用 |
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| 引用本文： | 高志强,宋立,张鹤晓,宁宜宝,乔彩霞,杨承槐,吴丹,谷强. 猪链球菌通用及2型TaqMan荧光PCR检测技术建立与应用[J]. 中国预防兽医学报, 2007, 29(7): 545-549 |
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| 作者姓名： | 高志强 宋立 张鹤晓 宁宜宝 乔彩霞 杨承槐 吴丹 谷强 |
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| 作者单位： | 1. 北京出入境检验检疫局,北京,100029
2. 中国兽医药品监察所,北京,100081 |
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| 摘 要： | 在对设计合成的引物和6-carboxy-fluorescein(6-FAM)荧光素标记的TaqMan水解探针进行筛选的基础上,通过反应体系优化,建立了检测猪链球菌通用和2型的2种荧光PCR检测技术,实现了对猪链球菌的快速检测和定型。敏感性、特异性、重复性、感染组织模拟试验及临床样品的检测结果均表明建立的方法快速、特异、灵敏,整个检测过程(包括样品的前处理时间)可在1.5 h内完成,检测培养物和模拟组织样品的灵敏度可达10 CFU～100 CFU。
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| 关 键 词： | 猪链球菌 荧光PCR |
| 文章编号： | 1008-0589（2007）07-0545-05 |
| 修稿时间： | 2005-09-17 |
Development and application of TaqMan real-time PCR for Streptococcus suis and S.suis type 2 |
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| GAO Zhi-qiang,SONG Li,ZHANG He-xiao,NING Yi-bao,QIAO Cai-xia,YANG Cheng-huai,WU Dan,GU Qiang. Development and application of TaqMan real-time PCR for Streptococcus suis and S.suis type 2[J]. Chinese Journal of Preventive Veterinary Medicine, 2007, 29(7): 545-549 |
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| Authors: | GAO Zhi-qiang SONG Li ZHANG He-xiao NING Yi-bao QIAO Cai-xia YANG Cheng-huai WU Dan GU Qiang |
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| Affiliation: | 1. Beijing Entry-exit Inspection and Quarantine Bureau, Beijing 100029, China; 2. China Institute of Veterinary Drug Control, Beijing 100081, China |
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| Abstract: | Two real-time PCR assays for the simultaneous detection of Streptococcus suis and S.suis type 2 were developed using selective primers and TaqMan hydrolysis type probes labeled with 6-carboxy-fluorescein(6-FAM)reporter dyes.The assays were examined for sensitivity,specificity,reproducibility for detection of mocked tissue and clinical samples.The results showed that both assays were rapid,specific and sensitive.The assays could be finished in 1.5 hours(including the pretreatment of sam- ples),and the detection threshold was 10 CFU-100 CFU when used to detect the cultures and mocked tissues. |
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| Keywords: | Streptococcus suis real-time PCR |
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