Nucleotide sequence of the coat protein gene of Mirafiori lettuce virus |
| |
| Authors: | Yoichi Kawazu Takahide Sasaya Toshiyuki Morikawa Keita Sugiyama Tomohide Natsuaki |
| |
| Institution: | (1) National Institute of Vegetable and Tea Science, Ano, Age, Mie 514-2392, Japan, JP;(2) National Agricultural Research Center for Western Region, Kagawa, Japan, JP;(3) Vegetable and Ornamental Crops Experiment Station, Toyama Agricultural Research Center, Tonami, Japan, JP;(4) Faculty of Agriculture, Utsunomiya University, Utsunomiya, Japan, JP |
| |
| Abstract: | The coat protein (CP) gene of Mirafiori lettuce virus (MiLV), a tentative member of the genus Ophiovirus was isolated and sequenced. The established sequence consists of 1514 nucleotides including one open reading frame (ORF)
with 1311 nucleotides that encodes 437 amino acids with a relative molecular mass 48 543. When the ORF was expressed in Escherichia coli, the obtained protein was confirmed as CP by Western blotting using an antiserum against MiLV. Database searches showed that
the CP gene of MiLV has a sequence similar to that of Citrus psorosis virus (CPsV), the type species of the genus Ophiovirus. The comparison between MiLV and CPsV CP genes revealed that the identities of the nucleotide and amino acid sequences were
46.5% and 30.9%, respectively.
Received: July 29, 2002 / Accepted: October 2, 2002 |
| |
| Keywords: | Mirafiori lettuce virus Ophiovirus Gene for coat protein |
| 本文献已被 SpringerLink 等数据库收录！ |
|
