The fungicides thiram and captan affect the phenotypic characteristics of Rhizobium leguminosarum strain C1 as determined by FAME and Biolog analyses |
| |
Authors: | K E Dunfield S D Siciliano J J Germida |
| |
Institution: | (1) Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, CA |
| |
Abstract: | We assessed the effects of the fungicides captan and thiram on the survival and phenotypic characteristics of Rhizobium leguminosarum bv. viceae, strain C1. Fungicide was applied to pea seed at rates of 0.25–2 g a.i. kg–1 seed, and treated seed was inoculated with strain C1. After 24 h, rhizobia were extracted from treated seed and viable numbers
determined by plating onto yeast extract mannitol agar. Phenotypic characteristics were assessed using FAME (fatty acid methyl
ester) profiles and Biolog substrate utilization patterns. Captan and thiram significantly reduced the numbers of rhizobia
recovered from seed and altered the FAME and Biolog profiles of recovered rhizobia. However, only the highest concentrations
of captan affected nodulation and plant growth. Contact with some seed-applied fungicides can significantly alter phenotypic
characteristics of rhizobia, but these changes might be offset by the presence of host plants.
Received: 20 February 1999 |
| |
Keywords: | Rhizobia Captan Thiram FAME Biolog |
本文献已被 SpringerLink 等数据库收录! |
|