| 胆碱磷酸转移酶基因转化大豆的初步研究 |
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| 引用本文： | 王桂玲,黄永芬.胆碱磷酸转移酶基因转化大豆的初步研究[J].大豆科学,2005,24(2):150-153. |
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| 作者姓名： | 王桂玲 黄永芬 |
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| 作者单位： | 哈尔滨师范大学生命科学系,哈尔滨,150080 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 胆碱磷酸转移酶基因(cpt)是磷脂合成代谢途径上一个关键的酶基因,其基因产物是磷脂酰胆碱(PC),PC做为主要膜脂与膜的流动性和植物的抗寒性有密切关系.本实验用花粉管通道法将质粒PRDH401(含35S-35S-AMV-cpt-NOST)导入五个黑龙江常见栽培大豆品种中,对D1代植株进行卡那霉素抗性筛选、低温筛选和PCR检测,初步筛选到转化植株.
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| 关 键 词： | 大豆 抗寒 CPT基因 转基因植物 |
| 文章编号： | 1000-9841(2005)02-0150-04 |
THE RESEARCH ON TRANSFERING CPT GENE INTO SOYBEAN |
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| Wang Guiling,Huang Yongfen.THE RESEARCH ON TRANSFERING CPT GENE INTO SOYBEAN[J].Soybean Science,2005,24(2):150-153. |
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| Authors: | Wang Guiling Huang Yongfen |
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| Abstract: | Cold resistance in the low temperature-induced plants is closely related to the synthesis metabolism path of phospholipids.Cholinephospho transferase gene is a key enzyme gene in the synthesis metabolism path of phospholipids.Phosphotidy choline(PC)is the enzyme gene product of CPT gene and it is the key component of the membrane..It is closely related with the mobility of membrane and cold resistance. In this experiment,plasmid PRDH401 which carrying the 35S-35S-AMV-cpt-NOST fusion gene,was transferred into 5 varieties of soybean by means of the pollen tube pathwa.We got the D-1 generation plants in the next year.Through the selection of kanamycin and low temperature,several transformed seedlings were obtained.PCR of the transformed soybean DNA indicated the integration of cpt gene into the transgenic soybean genome. |
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| Keywords: | Soybean Cold resistance CPT gene Transgenic plants |
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