In Vitro propagation of juvenileNothofagus leoni Espinosa (Fagaceae) |
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Authors: | Guillermo J Martínez Pastur Miriam E Arena |
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Institution: | (1) Centro Austral de Investigaciones Científicas, Consejo Nacional de Investigaciones Científicas y Técnicas. cc 92 (9410) Ushuaia, Tierra del Fuego, Argentina |
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Abstract: | Nothofagus leoni has a restricted distribution in Chilean forests. This work determines suitable culture conditions forin vitro multiplication and rooting through shoots obtained from seedlings. Broadleaved Tree Medium was suitable for shoot multiplication.
A medium with a pulse of 0.55 μM BA in the first subculture and two subcultures on BA-free medium resulted in a multiplication
rate at day 63 of × 5.7, without callus growth or shoot neoformation. Rooted shoots of good quality (number and length of
roots without callus growth) were obtained with 1.23 μM IBA (91.4% of rooting). The first roots appeared at day 11, reaching
a higher speed of rooting at day 15. |
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Keywords: | cytokinin pulse in vitro multiplication Nothofagus leoni rooting |
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