| 一种高效稳定的甘蓝染色体制片新方法 |
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| 引用本文： | 卢军,李乐玉,朱利泉,王小佳. 一种高效稳定的甘蓝染色体制片新方法[J]. 北方园艺, 2008, 0(9) |
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| 作者姓名： | 卢军 李乐玉 朱利泉 王小佳 |
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| 作者单位： | 西南大学,农学与生物科技学院,重庆,400716 |
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| 摘 要： | 介绍一种高效稳定的甘蓝根尖染色体制片新方法,该方法结合传统的压片法和去壁低渗法优点,制片取得了良好的结果:染色体中期分裂相多,染色体分散良好,形态特征清晰,背景干净.制片可应用于染色体核型分析、分带、荧光原位杂交,以及显微切割等细胞遗传学操作.
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| 关 键 词： | 甘蓝 染色体 制片 |
An Efficient and Stable Method For Chromosome Flaking in Brassica oleracea |
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| LU Jun,LI Le-yu,ZHU Li-quan,WANG Xiao-jia. An Efficient and Stable Method For Chromosome Flaking in Brassica oleracea[J]. Northern Horticulture, 2008, 0(9) |
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| Authors: | LU Jun LI Le-yu ZHU Li-quan WANG Xiao-jia |
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| Abstract: | An efficient and stable method for chromosome flaking of Brassica oleracea toot-tip has been developed.This method integrated the disadvantage of squashing method and wall digestion hypotonic method,the primary results showed that the modified process could easily get more metaphase cells,satisfying background,well decentraztion and clear morphological chromosome.these chromosomal specimens were fitted to be used in such cytogenetics manipulations as karyotyping,banding,fluorescence in situ hybridization and microdissection. |
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| Keywords: | Brassica oleracea Chromosome Flaking |
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