| 应用加端PCR克隆弓形虫P30基因 |
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| 引用本文： | 赵文忠,王祥生.应用加端PCR克隆弓形虫P30基因[J].中国兽医寄生虫病,1998,6(4):1-2. |
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| 作者姓名： | 赵文忠 王祥生 |
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| 作者单位： | 长春农牧大学兽医研究所寄生虫病研究室!130062 |
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| 摘 要： | 为了采用基因工程方法大量生产高效抗原提供技术，根据已发表的弓形虫RH株P30的核苷酸序列，设计了一对加端引物，运用PCR方法，从弓形虫NT株中扩增出约800bp的片段。产物经EcoRI和XbaI酶切后，克隆进PUC19载体，经酶切和PCR鉴定，插入片段为P30基因。
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| 关 键 词： | 弓形虫 加端PCR P30基因 |
CLONING P30 GENE BY PCR WITH ADDING END |
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| Zhao Wenzhong, Wang Xiangsheng, Liu Wancheng.CLONING P30 GENE BY PCR WITH ADDING END[J].Chinese Journal of Veterinary Parasitology,1998,6(4):1-2. |
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| Authors: | Zhao Wenzhong Wang Xiangsheng Liu Wancheng |
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| Institution: | Laboratory of Parasitology Veterinary Institute University of Agriculture and Animal Science of Changchun 130062 |
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| Abstract: | In order to provide technology to large production of highly effcient antigens bygene-project method. Based on the sequence of the major surface antigen gene of T. gondii published by Burg et al in 1993. Two primers were design and synthesized, the coding sequenceof P 30 was amplified from the T. gondii NT strain by PCR the amplified fragment wascloned into PUC 19 after cutted by EcoRI and Xbal. The recombinant was proved through digestion of enzymes and PCR. |
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| Keywords: | Toxoplasoma Gondii PCR with adding end P 30 gene |
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