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澳洲坚果叶斑病病原鉴定及其生物学特性
引用本文:柳凤,杨顺锦,詹儒林,赵艳龙. 澳洲坚果叶斑病病原鉴定及其生物学特性[J]. 植物保护学报, 2011, 38(5): 437-442
作者姓名:柳凤  杨顺锦  詹儒林  赵艳龙
作者单位:中国热带农业科学院南亚热带作物研究所,海南省热带园艺产品采后生理与保鲜重点实验室,湛江524091
基金项目:科技部农业科技成果转化资金项目(2008GB23260386),中央级非盈利性研究机构基本科研业务费研究专项(sscri201002), 海南省自然科学基金(808183)
摘    要:通过症状观察、致病性测定、形态和培养性状观察、rDNA-ITS序列分析及生物学活性测定将分离到的病原菌鉴定为异色拟盘多毛孢Pestalotiopsis versicolor(Speg.)Steyaert。菌株JG01在PDA培养基上菌落白色、背面呈橘黄色花瓣状,分生孢子盘墨汁状散生在菌丝上,分生孢子长梭形,大小为18.3~27.4μm×5.6~7.7μm,4隔,中间3个细胞着色,分隔处颜色很深,顶端附属丝一般2~3根。ITS序列分析,菌株JG01与P.versicolor(AF405298)亲缘关系最近,同源性达到99.60%。在PDMA培养基上,25~28℃、pH 4.0~6.0、碳氮源分别为葡萄糖和酵母提取物的条件下菌丝生长最快,培养条件对菌株产孢的影响不明显。

关 键 词:澳洲坚果  叶斑病  异色拟盘多毛孢  生物学特性
收稿时间:2011-01-13

Identification and biological characteristics of macadamia leaf spot caused by Pestalotiopsis versicolor
Liu Feng,Yang Shunjin,Zhan Rulin and Zhao Yanlong. Identification and biological characteristics of macadamia leaf spot caused by Pestalotiopsis versicolor[J]. Acta Phytophylacica Sinica, 2011, 38(5): 437-442
Authors:Liu Feng  Yang Shunjin  Zhan Rulin  Zhao Yanlong
Affiliation:South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Post-harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang 524091, Guangdong Province, China;South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Post-harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang 524091, Guangdong Province, China;South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Post-harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang 524091, Guangdong Province, China;South Asia Tropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Post-harvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang 524091, Guangdong Province, China
Abstract:
Keywords:macadamia  leaf spot  Pestalotiopsis versicolor  biological characteristics
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