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| 小麦叶片蛋白质双向电泳的改良方法 | |
| 引用本文： | 朱宏,张中恒,王同昌.小麦叶片蛋白质双向电泳的改良方法[J].东北林业大学学报,2004,32(4):68-69. |
| 作者姓名： | 朱宏 张中恒 王同昌 |
| 作者单位： | 1. 哈尔滨师范大学,哈尔滨,150080 2. 克山师范专科学校 3. 哈尔滨师范大学 |
| 基金项目： | 黑龙江省自然科学基金资助项目，哈尔滨师范大学博士科研启动基金资助项目 |
| 摘 要： | 以普通小麦叶片为实验材料，在原有双向电泳基础上通过对样品制备、样品溶解、等电聚焦电泳、SDS-聚丙烯酰胺凝胶电泳以及染色方法等关键步骤进行改进和优化，在小麦叶片可溶性蛋白的分析中获得了满意的双向电泳图谱。 |
| 关 键 词： | 双向电泳 等电聚焦电泳 SDS-聚丙烯酰胺凝胶电泳 |
An Improving Method of Two-D Gel Electrophoresis for Analyzing the Protein in Wheat Lamina |
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| Zhu Hong.An Improving Method of Two-D Gel Electrophoresis for Analyzing the Protein in Wheat Lamina[J].Journal of Northeast Forestry University,2004,32(4):68-69. | |
| Authors: | Zhu Hong |
| Abstract: | By using the lamina from Triticum aestivum as experimental material, a satisfied picture of two-D gel electrophoresis was obtained based on the analysis of its soluble protein through improving and optimizing the key procedures, such as sample preparation, sample dissolution, IEF electrophoresis, SDS-PAGE and dyeing routine. This research result will provide a foundation for primarily analyzing the protein in wheat and other plants. |
| Keywords: | Two-D gel electrophoresis IEF electrophoresis SDS-PAGE |
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