| 利用线粒体DNA(mtDNA)鉴别玉米各类不育胞质种子的研究 |
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| 引用本文： | 张亚兵,秦太辰.利用线粒体DNA(mtDNA)鉴别玉米各类不育胞质种子的研究[J].安徽农业科学,1998,26(3):195-197. |
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| 作者姓名： | 张亚兵 秦太辰 |
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| 作者单位： | [1]安徽省种子公司 [2]扬州大学农学院 |
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| 摘 要： | 提取黑暗中培养的玉米雄性不育胞质材料Ｔ群、Ｃ群、Ｓ群、ＹⅡ－１型和正常可育胞质的黄化苗体内的线粒体ＤＮＡ（ｍｔＤＮＡ），通过琼脂糖凝胶电泳分析未经酶切和酶切（限制性核酸内切酶分别为ｘｈｏＩ和ＢａｍＨＩ）的ｍｔＤＮＡ的电泳特性。发现：Ｔ群、Ｃ群、Ｓ群以及ＹⅡ－１型彼此ｍｔＤＮＡ组成上都存在差异。据此，可用ｍｔＤＮＡ鉴别玉米各类不育胞质种子。
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| 关 键 词： | 玉米 雄性不育胞质 线粒体DNA（mtDNA） |
Research on Identification of the Maize Seeds with Various Male Sterile Cytoplasm by Analysis of Mitochondrial DNA(mtDNA) |
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| Zhang Yabinget al.Research on Identification of the Maize Seeds with Various Male Sterile Cytoplasm by Analysis of Mitochondrial DNA(mtDNA)[J].Journal of Anhui Agricultural Sciences,1998,26(3):195-197. |
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| Authors: | Zhang Yabing |
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| Abstract: | Maize mitochondrial DNAs from Tgroup,C group,YⅡ Ⅰtgpe and normal cytoplasm N were prepared from the yellow seedling grown in the darkness.The undigested and digested(restriction endonuclease xho I and BamH I) mt DNAs were analysed by agrose gels electrophresis.The results showed that there were differences among T,C,S groups and YⅡ Ⅰ type.So we could idendify various male sterile cytoplasm with mitochondrial DNA in maize. |
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| Keywords: | Maize Male sterile cytoplasm Mitochondrial DNA |
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