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Hemagglutination-inhibition test applied to the study of akabane virus infection in domestic animals |
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| Authors: | Y Goto Y Inaba Y Miura H Kurogi E Takahashi K Sato T Omori T Hanaki H Sazawa M Matumoto |
| Institution: | 1. National Veterinary Assay Laboratory, Kokubunji, Tokyo, Japan;7. National Institute of Animal Health, Kodaira, Tokyo, Japan;71. Kyushu Branch Laboratory, National Institue of Animal Health, Kagoshima, Japan;77. Kitasato Institute, Minato-ku, Tokyo, Japan |
| Abstract: | Some factors affecting the hemagglutination-inhibition (HI) reaction with Akabane virus were investigated and an HI test developed. The test was proven to be useful in studies of antibody responses in cattle and other domestic animals infected with Akabane virus. HI antibody titers of individual animals were shown to be closely correlated with their neutralizing antibody titers and to remain undiminished for a relatively long time. In some early sera from domestic animals infected with Akabane virus, HI antibody sensitive to 2-mercaptoethanol was demonstrated. |
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