Phaseolus vulgaris-rhizobia symbiosis increases the phosphorus uptake and symbiotic N2 fixation under insoluble phosphorus |
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| Authors: | Abdi Neila Bargaz Adnane Faghire Mustapha Bouraoui Manel Hmissi Imen L’taief Boulbaba |
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| Institution: | 1. Programme des Légumineuses à Graines, Laboratoire des Grandes Cultures, Institut National de la Recherche Agronomique de Tunisie, Ariana, Tunisia;2. Faculté des Sciences de Bizerte, Université de Carthage, Bizerte, Tunisianeilaabdi@yahoo.fr;4. Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, Faculté des Sciences et Techniques Guéliz-Marrakech, Marrakech, Morocco;5. Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, Faculté des Sciences et Techniques Guéliz-Marrakech, Marrakech, Morocco;6. Faculté des Sciences de Bizerte, Université de Carthage, Bizerte, Tunisia;7. Institut National d’Agronomie de Tunis, Tunis, Tunisia |
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| Abstract: | Five north Tunisian native rhizobia were tested in symbiosis with ‘Coco-blanc’ variety under two phosphorus (P) supplies P-sufficient and insoluble P (IP)] at 250 μmol P plant?1. At flowering stage, responses against IP were assessed and indicated that inoculation with P.Ps.09 produced a high nodule biomass (0.12g). Similarly, P.Tb.09 produced 0.08 g nodule biomass under both P and P.Bj.09 that significantly increased nodule number under IP. Shoot biomass exhibited high dry weight with P.Tb.09 and CIAT899 (1.6 g plant?1). Inoculation with CIAT899 produced a high root biomass under both P. Analysis of a halo diameter of clear zone rounding each colony under IP indicated that P.Tb.09 has developed high halo Ø (1.7cm). These variations were associated under IP with a decrease of pH by P.Ar.09 and with increase in shoot phosphatase activity by P.Ar.09, P.Bj.09, P.OM.09 and P.Ps.09. The use of P-efficient rhizobia may constitute an adaptive mechanism against P-deficiency tolerance. |
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| Keywords: | insoluble phosphorus nitrogen Phaseolus vulgaris acid phosphatase rhizobia |
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