| 新生小鼠卵巢组织玻璃化冷冻保存与异体异位移植研究 |
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| 引用本文： | 廖海艳,周卫国,王方剑. 新生小鼠卵巢组织玻璃化冷冻保存与异体异位移植研究[J]. 动物医学进展, 2010, 31(9) |
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| 作者姓名： | 廖海艳 周卫国 王方剑 |
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| 作者单位： | 湖南省第一师范学院,湖南长沙,410205;湖南省永州零陵区畜牧水产局动物防疫监督站,湖南永州,425006 |
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| 基金项目： | 湖南省大学生研究性学习和创新性实验计划项目 |
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| 摘 要： | 开展小鼠新生胎儿卵巢组织冷冻保存与移植研究,为建立家畜卵巢组织冷冻保存与移植技术奠定基础。采用微滴法玻璃化冻存方法处理卵巢,卵巢复苏后移植到昆明系雄性小鼠受体肾囊下,19只雄性受体鼠接受了卵巢移植,每侧肾囊移植2枚,卵巢共移植1日龄小鼠卵巢38个。饲喂28 d有效回收移植卵巢的受体鼠为17只,受体总有效率分别为89.4%;有效回收移植卵巢30个,卵巢总回收率为78.9%。结果表明,小鼠早期卵巢经过冷冻、解冻并异体异位移植后,其原始卵泡能够重新启动生长发育。
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| 关 键 词： | 小鼠 玻璃化冷冻保存 卵巢移植 |
Study on Allograft and Vitrification of Newborn ouse Ovarian Tissue in Male Recipient Mice |
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| LIAO Hai-yan,ZHOU Wei-guo,WANG Fang-jian. Study on Allograft and Vitrification of Newborn ouse Ovarian Tissue in Male Recipient Mice[J]. Progress In Veterinary Medicine, 2010, 31(9) |
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| Authors: | LIAO Hai-yan ZHOU Wei-guo WANG Fang-jian |
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| Affiliation: | LIAO Hai-yan1,ZHOU Wei-guo2,WANG Fang-jian3(1.Hunan First Normal College,Changsha,Hunan,410205,China,2.Animal Epidemic Prevention Supervision Station,Yongzhou,425006,China) |
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| Abstract: | The cryopreservation and transplantation of ovarian tissue is considered as one of new technique to build mammal ovum library in the future.To explore methods of vitrification of ovarian tissue and the ideal cryopretectants and build the cryopreservation and transplantation technology of livestock ovary tissue,the vitrified ovarian tissue of newborn mice was allografted underneath the kidney capsule of male recipients in the experiment.1-day-old mouse ovarian tissue were theated by the cryovial method vitri... |
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| Keywords: | mice vitrification ovary transplantation |
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