| PRSV-CP基因小hpRNA植物表达载体的构建 |
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| 引用本文： | 王树昌,言普,沈文涛,周鹏. PRSV-CP基因小hpRNA植物表达载体的构建[J]. 热带作物学报, 2009, 30(2): 176-180 |
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| 作者姓名： | 王树昌 言普 沈文涛 周鹏 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 国家自然科学基金项目(No.30760134);;中央级科研院所基本科研业务费项目资助。 |
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| 摘 要： | 将获自海南(prsv-cp hno1～4)、云南(prsv-cp yunnan)和广西(prsv-cp guanxi)的番木瓜环斑病毒株系外壳蛋白(PRSV-CP)基因序列与DQ340771、X97251(来自中国台湾的PRSV株系)以及AY162218、AY231130、DQ374153、S46722、X97251(分别来自泰国、墨两哥、巴西、美国的PRSV株系)等外壳蛋白(CP)基因序列进行比对,在CP基因高保守区设计靶向目标序列进行亚克隆,获得长度为50bp的DNA片段,并通过一步套叠PCR把50 bp 正向片段和反向片段与拟南芥内含子拼接,成功构建了小hpRNA结构的植物表达载体,为进一步培育安全性好、抗谱广的番木瓜转基因新种质奠定了基础.
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| 关 键 词： | CP基因 植物表达载体 |
Construction of hpRNA Plant Expression Vectors for PRSV-CP Gene |
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| Wang Shuchang,Yan Pu,Shen Wentao and Zhon Peng. Construction of hpRNA Plant Expression Vectors for PRSV-CP Gene[J]. Chinese Journal of Tropical Crops, 2009, 30(2): 176-180 |
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| Authors: | Wang Shuchang Yan Pu Shen Wentao Zhon Peng |
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| Affiliation: | Wang Shuchang,Yan Pu,Shen Wentao,Zhon Peng Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou,Hainan 571101,China |
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| Abstract: | Through aligning PRSV-CP genes in China and those in other countries or regions, 50 bp DNA fragment in high conserved region of PRSV-CP gene were amplified from PRSV strains in Hainan, Yunnan and Guangxi. Using one -step overlap extension PCR, the 50 bp sense fragment and antisense fragment of PRSV-CP were spliced with the Arabidopsis thialiana intron fragment and constructed into plant expression vector pBI121 and pCAMBIA3300. This study could facilitate to obtaining of new transgenic papaya varieties with... |
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| Keywords: | PRSV hpRNA |
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