山梨醇螯合钙对NaCl胁迫下油菜种子萌发的影响

本文通过控制钙源种类(螯合态钙和游离态钙)、两种形态钙配比(不同螯合率)及螯合态钙浓度等3组试验探究山梨醇螯合钙对NaCl胁迫下油菜种子萌发的影响.结果表明:当培养液中NaCl浓度为150或170 mmol/L时严重影响油菜种子的萌发和胚根生长,添加两种形态钙后均能显著提高NaCl胁迫下种子活力和发芽率,促进胚根的生长...

Effect of Sorbitol Chelated Calcium on Seed Germination of Rapeseed Under NaCl Stress

In this paper, the effects of sorbitol chelated calcium on rape seed germination under NaCl stress were studied by controlling the types of calcium source (chelated calcium and free calcium), the mixture ratio of two forms of calcium (different chelation rate) and the concentration of chelated calcium. The results showed that when the concentration of NaCl in the culture medium was 150 or 170 mmol/L, it seriously affected the germination and radicle growth of rape seeds. The addition of two forms of calcium could significantly improve the seed vigor and germination rate under NaCl stress, promote the growth and elongation of radicle. Under NaCl stress (the concentration is 170 mmol/L), the addition of sorbitol chelated calcium with different chelation rates can significantly increase the germination potential and germination rate of rape seeds, and the promotion order is 70% > 0 > 40% > 10% > 100%. When the proportion of chelated calcium is 70%, the effect of promoting seed germination is better than single calcium nitrate or entire chelated calcium. Under NaCl stress (the concentration was 170 mmol/L), the addition of different concentrations of chelated calcium could reduce the salt damage, and the seed germination potential of sorbitol chelated calcium which Ca2+ concentration is 0.897 g/L was the largest; at the end of germination, the radicle length of seedlings treated with sorbitol chelated calcium which Ca2+ concentration is 0.577 g/L was the longest. When the Ca2+ concentration was further increased, the promotion effect would be reduced. In conclusion, soaking seeds with sorbitol chelated calcium under salt stress can reduce salt damage. The coexistence of chelated calcium and free calcium in the ratio of 7:3 has the best effect on promoting rape seed germination under salt stress, and low concentration of chelated calcium has a better effect on promoting rape seed germination under salt stress.