| 番茄白粉菌的PCR分子检测 |
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| 引用本文： | 王文静,李成伟.番茄白粉菌的PCR分子检测[J].河南农业科学,2010(5). |
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| 作者姓名： | 王文静 李成伟 |
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| 作者单位： | 1. 商丘师范学院,生命科学系,植物与微生物互作重点实验室,河南,商丘476000
2. 商丘师范学院,生命科学系,植物与微生物互作重点实验室,河南,商丘476000;周口师范学院,生命科学系,植物遗传与分子育种重点实验室,河南,周口466001 |
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| 基金项目： | 国家自然科学基金，教育部科学技术研究重点项目，河南高校杰出人才科研创新工程项目，留学回国人员科研启动基金 |
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| 摘 要： | 根据番茄白粉菌核糖体DNA的ITS(internal transcribed spacer)序列的特异性,设计2对引物OidF1/R1、OidF2/R2,对番茄白粉菌进行了PCR特异性扩增试验。结果表明:2对引物都可以从番茄白粉菌基因组DNA中扩增出350 bp左右的特异性扩增产物,并具有专一性,只在白粉菌和接种感病组织DNA中有扩增条带,而番茄早疫病菌、叶霉菌以及健康组织DNA中均无特异性扩增片段;引物具有高度的灵敏度,可以检测含有1 ng的番茄白粉菌DNA。由此证明,该方法可快速、准确和灵敏地鉴定和检测番茄白粉病的发生。
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| 关 键 词： | 番茄白粉菌 PCR 分子检测 |
PCR Detection of Oidium neolycopersici |
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| WANG Wen-jing,LI Cheng-wei.PCR Detection of Oidium neolycopersici[J].Journal of Henan Agricultural Sciences,2010(5). |
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| Authors: | WANG Wen-jing LI Cheng-wei |
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| Abstract: | Based on the internal transcribed spacer(ITS) sequence difference between Oidium neolycopersici and other tomato fungal pathogens,two primer pairs(OidF1/R1,OidF2/R2) were designed to detect the fungal pathogen,tomato powdery mildew.For both pairs,specific bands around 350 bp were amplified respectively using genomic DNA extracted from O.neolycopersici,inoculated tomato leaf while no PCR product was amplified with DNA from other fungi inoculaterd and noninoculated tomato leaves.As low as 1 ng of O.neolycopersici genomic DNA can be detected by both specific primer pairs.This protocol provides a rapid,reliable and sensitive tool for detection and identification of O.neolycopersici. |
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| Keywords: | PCR |
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