Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field |
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Authors: | Dispas M Schynts F Lemaire M Letellier C Vanopdenbosch E Thiry E Kerkhofs P |
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Institution: | Department Biocontrol/Section Epidemiology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium. |
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Abstract: | During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE-negative live-attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and PCR. All the techniques used identified the isolated virus as a gE-negative BHV-1 phenotypically and genotypically identical to the Za strain used as a control. |
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