| 扁桃SSR反应体系的优化 |
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| 引用本文： | 吴婷,高疆生,吴瑾,乔霞,胡学林.扁桃SSR反应体系的优化[J].西北农业学报,2008,17(3):219-222,241. |
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| 作者姓名： | 吴婷 高疆生 吴瑾 乔霞 胡学林 |
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| 作者单位： | 1. 塔里木大学植物科技学院,新疆阿拉尔,843300;塔里木大学新疆兵团塔里木盆地生物资源保护与利用重点实验室,新疆阿拉尔,843300
2. 兵团农二师三十三团,新疆库尔勒,841000 |
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| 基金项目： | 兵团科技计划应用基础研究项目 |
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| 摘 要： | 以南疆部分扁桃品种为试材,利用CTAB法提取基因组DNA,将PCR的主要成分设定4个梯度,优化了扁桃SSR技术中PCR反应体系,试验结果表明,适宜扁桃SSR技术体系为:在20μL反应体系中TaqDNA聚合酶和DNA最适用量分别为1U和100 ng;Mg2 、dNTP和引物最适终浓度分别为1.5 mmol/L、0.20 mmol/L和0.2μmol/L。利用18个扁桃品种验证此反应体系,非变性聚丙烯酰胺凝胶电泳检测结果显示,扩增产物大多在100~300 bp,多态性高,且反应体系的稳定性和可重复性好。
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| 关 键 词： | 扁桃 SSR 优化 |
| 收稿时间： | 2007/11/28 0:00:00 |
| 修稿时间： | 2007/12/20 0:00:00 |
Optimization of SSR Reaction System in Almond |
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| WU Ting,GAO Jiang-sheng,WU Jin,QIAO Xia and HU Xue-lin.Optimization of SSR Reaction System in Almond[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(3):219-222,241. |
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| Authors: | WU Ting GAO Jiang-sheng WU Jin QIAO Xia and HU Xue-lin |
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| Institution: | College of Plant Science and Technology, Tarim University, Alar Xinjiang 843300, China;Xinjiang Production & Construction Corps Key Laboration of Protection and Utilization of Biological Resources in Tarim Basin Tarim University, Alar Xinjiang 8;College of Plant Science and Technology, Tarim University, Alar Xinjiang 843300, China;Xinjiang Production & Construction Corps Key Laboration of Protection and Utilization of Biological Resources in Tarim Basin Tarim University, Alar Xinjiang 8;College of Plant Science and Technology, Tarim University, Alar Xinjiang 843300, China;Xinjiang Production & Construction Corps Key Laboration of Protection and Utilization of Biological Resources in Tarim Basin Tarim University, Alar Xinjiang 8;College of Plant Science and Technology, Tarim University, Alar Xinjiang 843300, China;Xinjiang Production & Construction Corps Key Laboration of Protection and Utilization of Biological Resources in Tarim Basin Tarim University, Alar Xinjiang 8 |
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| Abstract: | |
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| Keywords: | Almond SSR Optimization |
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