水貂出血性肺炎大肠杆菌毒力基因测定和H基因分型

为了分离鉴定引起水貂出血性肺炎的大肠杆菌,并对其致病性、血清型和毒力基因进行鉴定.本研究主要通过细菌分离鉴定试验对具有典型肺炎症状的死亡水貂的病原体进行分离,利用16S rRNA对进行细菌鉴定,并通过PCR方法对分离细菌的血清型和毒力基因进行检测,分析分离菌对动物的致病性.结果显示:28例水貂肺炎病例中分离出8株大肠杆...

Virulence Gene Detection and H Genotyping of Escherichia coli from Mink Hemorrhagic Pneumonia

In order to isolate and identify Escherichia coli strains causing mink hemorrhagic pneumonia and to further study their pathogenicity,serotypes and virulence genes,tissue samples were collected from dead minks with typical pneumonia for bacterial isolation and identifi cation.As a result,8 Escherichia coli strains were isolated from 28 mink pneumonia cases.These 8 E.coli strains were identifi ed by 16S rRNA and PCR for testing their serotypes and virulence genes.The results showed that the H genotypes of these E.coli strains were H5,H11,H16 and H25,among which H11 was the main genotype accounting for 50%(4/8).There were 4 major virulence genes of E.coli including iutA,papC,irp2 and fyuA.Further testing revealed that H11 serotype strains contained 3 major virulence genes,iutA,irp2 and fyuA.The results from this study indicated that the main serotype of E.coli causing mink pneumonia was H11 and the main virulence genes were iutA,papC,irp2 and fyuA.