不同修复植被类型的三峡库区消落带土壤细菌群落分析

为了探究三峡库区消落带不同修复植被对土壤细菌群落结构的影响,以纯草地(A₁)、纯林地(A₂)和覆草林地(A₃)3种不同样地土壤为研究对象,采用土壤理化因子检测及扩增子测序的研究方法,分析了不同修复植被对三峡库区消落带土壤细菌群落的影响。结果表明:不同样地的pH值、全氮(TN)和全磷(TP)含量无显著性差异,A₁和A₃的土壤有机碳(SOC)含量之间无显著性差异,但均显著高于A₂(p<0.05); 相关分析显示样地土壤细菌群落ACE指数和Shannon指数与土壤SOC和TN含量显著正相关(p<0.05),Chao1指数与土壤SOC含量显著正相关(p<0.05); 不同样地土壤的细菌优势种群无明显区别; 属水平上6个优势种群的相对丰度与土壤SOC和TN含量显著相关(p<0.05); 冗余分析显示SOC是影响三峡库区消落带不同修复植被样地细菌群落的主要显著性因子(p<0.05)。由此可以得出,不同修复植被类型的消落带土壤的细菌优势种群无明显差异,土壤细菌群落结构主要受土壤SOC的影响。

Analysis of Soil Bacterial Communities in Water Level Fluctuating Zone of Three Gorges Reservoir Area with Different Re-vegetation Types

In order to study the effect of different re-vegetation types on soil bacterial communities in the water level fluctuating zone of the Three Gorges Reservoir area,three different soil samples,including pure grassland(A₁),pure woodland(A₂)and covered woodland(A₃),were used for physical and chemical factor detection and 16S rRNA gene sequencing,and the effects of different re-vegetation types on soil bacterial communities in the fluctuating zone of the Three Gorges Reservoir area were analyzed.The results showed that there were no significant differences in the values of pH value,total nitrogen(TN)and total phosphorus(TP)between different types of soil samples;there was no significant difference between the contents of soil organic carbon(SOC)of A₁ and A₃,which was significantly higher than A₂(p<0.05).Correlation analysis showed that the ACE index and Shannon index of soil bacterial community were significantly positively related to soil SOC and TN content(p<0.05),Chao1 index was significantly positively related to soil SOC content(p<0.05).There was no significant difference in the dominant bacterial populations in different soils.The relative abundance of each of the six dominant populations of bacteria at genus level was significantly related to soil SOC and TN contents,respectively(p<0.05).Redundancy analysis showed that SOC was the mainly dominant significant factor of soil bacterial community structure(p<0.05).It is shown that there is no significant difference in the dominant populations of soil bacteria under different re-vegetation types in Water Level Fluctuating Zone,and the structure of soil bacterial community is mainly influenced by soil SOC.