| 基于iTRAQ定量蛋白质组学技术的不同感染状态结核病牛血清蛋白质组学分析 |
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| 引用本文： | 房立春,高新桃,林伟东,贾红,郭晓宇,侯邵华,姜一曈,朱鸿飞,鑫婷.基于iTRAQ定量蛋白质组学技术的不同感染状态结核病牛血清蛋白质组学分析[J].中国动物传染病学报,2022(1):30-38. |
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| 作者姓名： | 房立春 高新桃 林伟东 贾红 郭晓宇 侯邵华 姜一曈 朱鸿飞 鑫婷 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所;中国农业科学院生物技术研究所;让布卢农学院比利时烈日大学 |
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| 基金项目： | 国家重点研发计划(2017YFD0500900,2016YFD0500902);国家自然科学基金项目(31602086);北京市自然科学基金(6164039)。 |
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| 摘 要： | 为了探明不同感染状态结核病牛血清蛋白质水平的差异,揭示结核病牛在不同感染状态下的生理状态.本研究通过皮内变态反应、IFN-γ释放试验从临床筛选结核病阳性牛(TB)和阴性牛(NC),根据牛鼻拭子巢式PCR检测结果将临床阳性牛(TB)分为PCR阳性结核病牛(TBP)和PCR阴性结核病牛(TBN),每组20头,采集血清.应用...
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| 关 键 词： | 牛结核病 感染状态 血清 差异蛋白 iTRAQ技术 |
Proteomics Analysis of Serum Samples from Cattle with Different Tuberculosis Infection Status Based on iTRAQ and Parallel Reaction Monitoring Techniques |
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| FANG Lichun,GAO Xintao,LIN Weidong,JIA Hong,GUO Xiaoyu,HOU Shaohua,JIANG Yitong,ZHU Hongfei,XIN Ting.Proteomics Analysis of Serum Samples from Cattle with Different Tuberculosis Infection Status Based on iTRAQ and Parallel Reaction Monitoring Techniques[J].Chinese Journal of Animal Infectious Diseases,2022(1):30-38. |
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| Authors: | FANG Lichun GAO Xintao LIN Weidong JIA Hong GUO Xiaoyu HOU Shaohua JIANG Yitong ZHU Hongfei XIN Ting |
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| Institution: | (Institute of Animal Science,CAAS,Beijing 100193,China;Biotechnology Research Institute,CAAS,Beijing 100081,China;Molecular and Cellular Biology,Gembloux Agro-Bio Tech University of Liège(ULg),Gembloux 4000,Belgium) |
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| Abstract: | The purpose of this study was to analyze proteomics in serum samples of cattle with different tuberculosis status.Tuberculosis positive cattle(TB,n=40)and healthy cattle(NC,n=20)were selected by the skin test(ST)and IFN-γrelease assay(IGRA).According to PCR testing for nasal swabs,40 TB cattle were then divided into PCR positive group(TBP,n=20)and PCR negative group(TBN,n=20).Serum samples were collected from all of these cattle and differential proteins were screened by isobaric tag for relative and absolute quantitation(iTRAQ)/mass spectrometry(MS).Besides,6 differential proteins were selected for parallel reaction monitoring(PRM)target verifi cation.The results showed that a total of 223 differentially expressed proteins were screened between TB and NC groups(FDR≥1.67 or≤0.67 and P≤0.05),among which 112 proteins were up-regulated and 111 proteins were down-regulated.A total of 74 differentially expressed proteins were selected between TBP and TBN groups,among which 46 proteins were up-regulated and 28 proteins were down-regulated.Parallel reaction monitoring was successfully quantitated to 6 validated proteins and the trend was consistent with iTRAQ.Bioinformatics analysis also indicated that the complement system and coagulation system signifi cantly changed after Mycobacterium bovis infection.The results of this study demonstrated that the great difference in serum protein compositions and physiological states existed among TB cattle with different infection statuses,which provided theoretical support for the distinction of different infection states of bovine tuberculosis.Therefore,the present study provided useful information for the prevention and control of bovine tuberculosis. |
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| Keywords: | Bovine tuberculosis infection status serum differential protein iTRAQ technique |
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