济宁青山羊生后发育阶段GnRH与GnRHR阳性细胞在下丘脑分布规律的研究

为研究济宁青山羊生后发育阶段GnRH及GnRHR在下丘脑内的形态学分布和变化规律,采用链霉亲和素-生物素-过氧化物酶复合物(Strept Avidin Biotin-Peroxidase Complex,SABC)免疫组织化学方法,对0、2、4和6月龄雌性济宁青山羊下丘脑中GnRH及GnRHR的分布进行了同步研究。结果显示,GnRH和GnRHR免疫阳性细胞在下丘脑内广泛存在,主要分布于视前内侧区、乳头体、视上核和视交叉上核。随月龄增长,GnRH和GnRHR阳性细胞不断增大,数量不断增多,其中0～2月龄是最快的时期。结果提示,下丘脑分泌的GnRH及GnRHR对生后发育阶段青山羊性成熟的启动及维持有重要作用。

Distribution Patterns of GnRH and GnRHR Positive Cells in Hypothalamus at Different Postnatal Stages in Jining Gray Goats

In order to detect the morphological changes and distribution patterns of GnRH and GnRHR in hypothalamus at different postnatal stages in Jining Gray goats,immunohistochemical Strept Avidin Biotin-Peroxidase Complex(SABC) three-step method was used to study the distribution of GnRH and GnRHR in hypothalamus in goats at 0,2,4 and 6 months old.The results showed that GnRH and GnRHR positive cells were widely scattered in hypothalamus.Most of the positive cells were distributed in medial preoptic area(MPA),mamillary nucleus(MB),supraoptic nucleus(SON) and suprachiasmatic nucleus(SCN).The size and number of positive cells were increased with the increase of month old.The numbers of positive GnRH and GnRHR cells were significantly higher at 2 month old(puberty) than that at birth.The results suggest that GnRH and GnRHR secreted by hypothalamus might play a pivotal role in the onset of puberty and the maintenance of maturation in Jining Gray goat at the postnatal developing stage.