丽蝇蛹集金小蜂P450基因家族的发现和分子进化分析

丽蝇蛹集金小蜂是一类重要的天敌昆虫，大量地应用于生物防治中。虽然寄生蜂不是化学农药的直接作用靶标，但其在自然环境中可能接触到化学农药。P450基因家族是昆虫产生抗药性的重要原因之一，同时也参与了昆虫激素的合成与降解。因此，研究P450基因家族对充分利用丽蝇蛹集金小蜂，以及保护其免受农药毒杀具有重要的意义。本文利用生物学信息方法从丽蝇蛹集金小蜂基因组筛选到91个P450基因。家族分析表明，丽蝇蛹集金小蜂具有15个P450基因超家族、27个亚家族。对P450基因的正选择和基因转换开展了进一步分析，研究发现5个基因族存在正选择位点，2个基因族存在基因转换，正选择和基因转换之间存在明显的关联性。各个亚家族的选择压力分析表明，CYP9P亚家族受到选择压力最大（ω=118．9355）。通过MEME程序在丽蝇蛹集金小蜂P450基因上识别到5个频率为90％以上的motif，其中“LAENH]P”为新发现的motif.

Genome-level Identification and Evolution Analysis of P450 Gene Family in Nasonia vitripennis

Nasonia vitripennisis is an important class of natural enemies of pests, which has been widely used in biological control. Though N. vitripennisis is not the direct target of insecticides, it is still likely to get in touch with insecticides in wide environment. P450 gene family is not only an important factor conferring insecticide resistance but also participates in synthesis and degradation of hormones. Therefore, investigation of P450 genes in N. vitripennisis is great helpful for fully using in biological control and protects it from insecticides. We identified 91 P450 genes from the genome of N. vitripennis. Gene family analysis demonstrated that there were 15 P450 super gene families and 27 subfamilies in iV. vitripennis. We also found that five P450 gene groups meet positive selection and two gene groups have gene conversion. There is a positive relation between selection and gene conversion. The results indicated that CYP9P had the highest selection pressure. We also identified five motifs in N. vitripennis P450 gene family using MEME program. The occurrence frequencies of these five motifs were〉90%. The ＂LAENH]P＂ was a new motif identified in N. vitripennis P450 gene family.