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蚕沙不同溶剂萃取物的抑菌活性
引用本文:吴联,李定刚,周乐,张武岗,冉晓娅,杨明利. 蚕沙不同溶剂萃取物的抑菌活性[J]. 西北农业学报, 2006, 15(6): 212-214,220
作者姓名:吴联  李定刚  周乐  张武岗  冉晓娅  杨明利
作者单位:1. 西北农林科技大学生命科学学院,陕西杨凌,712100
2. 青岛康地恩实业有限公司,山东青岛266061
摘    要:采用石油醚、氯仿、乙酸乙酯和正丁醇依次对蚕沙70%乙醇粗提物进行萃取,对不同溶剂萃取物的化学成分进行预试,采用药敏纸片法测定了不同萃取物分别对停乳链球菌、金黄色葡萄球菌、无乳链球菌、乳房链球菌、大肠杆菌、沙门氏菌的抑菌活性。结果表明,各萃取物的抑菌活性由强到弱的顺序为;乙酸乙酯相〉石油醚相〉氯仿相〉正丁醇相,主要抑菌活性成分为香豆素/内酯和黄酮类/酚类。

关 键 词:蚕沙  抑菌活性  萃取物  成分预试
文章编号:1004-1389(2006)06-0212-03
收稿时间:2006-03-27
修稿时间:2006-03-272006-04-29

Antibacterial Activity of the Different Extracts from Silkworm Feces
WU Lian,LI Ding-gang,ZHOU Le,ZhANG Wu-gang,RAN Xiao-ya and YANG Ming-li. Antibacterial Activity of the Different Extracts from Silkworm Feces[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(6): 212-214,220
Authors:WU Lian  LI Ding-gang  ZHOU Le  ZhANG Wu-gang  RAN Xiao-ya  YANG Ming-li
Affiliation:College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China;Qingdao Continent Industry Co.LTD, Qingdao Shandong 266061, China;College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China;College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China;College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China;College of Life Science, Northwest A & F University, Yangling Shaanxi 712100, China
Abstract:The extract of 70% ethanol from Silkworm feces had been extracted by petroleum ether,chloroform,ethyl acetate and n-butanol orderly.And the component of different extracts had been tested.Antibacterial activity of different extractions from Silkworm feces was studied in this paper against S.aureas,S.agalactiae,S.uberis,S.dysagalactiae,E.col and Salmonella in vitro.The results showed that the antibacterial activity of extracts was decreased like the sequence: ethyl acetate extract>petroleum ether extract>chloroform extract>n-butanol extract.And the results showed that the main antibacterial activity components were lactones/cumarins and flavanoids/ phenols.
Keywords:Silkworm feces   Antibacterial activity   Extract   Component test
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