Expression of Interferon-tau mRNA in Bovine Embryos Derived from Different Procedures |
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| Authors: | N Yao P-C Wan Z-D Hao F-F Gao L Yang M-S Cui Y Wu J-H Liu S Liu H Chen S-M Zeng |
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| Institution: | Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing;;Reproductive Medical Center, Renji Hospital of Shanghai Jiao Tong University, Shanghai;;Animal Husbandry and Vet Institute, Xinjiang Academic of Agriculture and Reclamation Science, Shihezi, Xinjiang, China |
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| Abstract: | Interferon-tau (IFN-τ) is a secreted conceptus protein which plays a critical role in the establishment of ruminant pregnancy by its antiluteolytic and antiviral effects. In the present study, we hypothesized that IFN-τ expression was temporally and spatially regulated in different pre-implantation embryos and the levels of IFN-τ expression were different among bovine embryos derived from parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). By using in situ hybridization with Digoxingenin (DIG)-labelled IFN-τ cDNA as a probe, we detected IFN-τ mRNA in bovine embryos from days 3 to 9 in culture. However, the timing of the initiation of IFN-τ mRNA expression was different among PA, IVF and SCNT embryos. Interferon-τ mRNA was first expressed in 16-cell stage IVF embryos on day 4, in SCNT morula on day 5 and early PA blastocyst on day 6. Semi-quantitative RT-PCR analysis showed that the expression levels of IFN-τ mRNA did not differ significantly among IVF, SCNT and PA embryos on day 7. In addition, freezing and thawing did not have a major impact either on IFN-τ mRNA expression in IVF or in vivo -produced bovine blastocysts. |
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