| SOE法对大豆苯丙氨酸解氨酶(PAL)基因拼接的应用研究 |
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| 引用本文： | 宋柬,马会勤,陈尚武.SOE法对大豆苯丙氨酸解氨酶(PAL)基因拼接的应用研究[J].大豆科学,2006,25(3):228-233. |
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| 作者姓名： | 宋柬 马会勤 陈尚武 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学果树学系,北京,100094 |
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| 摘 要： | 利用SOE(Splicing by Overlap Extension)法对大豆苯丙氨酸解氨酶(PAL)的两个外显子进行拼接,得到完整的PAL基因,表明这种方法是有效可行的.这种技术是作为对cDNA克隆的初步改进与尝试,节约了成本,有一定的应用价值.
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| 关 键 词： | SOE法 cDNA克隆 苯丙氨酸解氨酶(PAL) |
| 文章编号： | 1000-9841（2006）03-0228-06 |
| 收稿时间： | 2006-03-23 |
| 修稿时间： | 2006-03-23 |
APLLICATION OF GENE SPLICING BY OVERLAP EXTENSION ON Glycine max PHENYLALANINE AMMONIA LYASE (PAL) |
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| Song Jian,Ma Huiqin,Chen Shangwu.APLLICATION OF GENE SPLICING BY OVERLAP EXTENSION ON Glycine max PHENYLALANINE AMMONIA LYASE (PAL)[J].Soybean Science,2006,25(3):228-233. |
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| Authors: | Song Jian Ma Huiqin Chen Shangwu |
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| Abstract: | The two exons of Glycine max phenylalanine ammonia lyase(PAL) were recombined together by gene splicing by overlap extension(gene SOEing).Results showed that gene SOEing was a successful specific PCR technique for gene recombination.It was the improvement of cloning cDNA. |
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| Keywords: | SOE cDNA Phenylalanine ammonia lyase(PAL) |
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