| 花生果种皮特异表达基因候选片段的分离 |
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| 引用本文： | 蔡宁波,张君诚,兰岚,庄伟建,赵永莉,李毓.花生果种皮特异表达基因候选片段的分离[J].福建农林大学学报(自然科学版),2007,36(6):596-598. |
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| 作者姓名： | 蔡宁波 张君诚 兰岚 庄伟建 赵永莉 李毓 |
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| 作者单位： | 福建农林大学油料作物研究所,福建,福州,350002 |
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| 摘 要： | 应用改良的琼脂糖凝胶电泳展示DDRT-PCR产物的方法分离花生果种皮特异表达基因,共分离出24条差异表达片段.经测序后排除重复,最终得到8条差异片段.其中1条为花生上已知基因片段,余下7条均为花生上尚未报道过的基因.此7个片段序列已登录Genbank,其登录号分别为DQ450065、DQ450066、DQ450067、DQ450068、DQ450069、DQ450070、DQ450071.同时本研究还对应用琼脂糖电泳分离DD-PCR产物的方法进行了探讨.
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| 关 键 词： | 花生 果种皮 特异基因 反转录差异显示技术 |
| 文章编号： | 1671-5470(2007)06-0596-03 |
| 修稿时间： | 2007年1月15日 |
Candidate fragments isolating of pod and testa specifically expressed genes in peanut |
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| CAI Ning-bo,ZHANG Jun-cheng,LAN Lan,ZHUANG Wei-jian,ZHAO Yong-li,LI Yu.Candidate fragments isolating of pod and testa specifically expressed genes in peanut[J].Journal of Fujian Agricultural and Forestry University,2007,36(6):596-598. |
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| Authors: | CAI Ning-bo ZHANG Jun-cheng LAN Lan ZHUANG Wei-jian ZHAO Yong-li LI Yu |
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| Abstract: | Appling improved method for isolating DDRT-PCR products using agarose gel electrophoresis,24 differentially expressed cDNA fragments were isolated from the RNA of peanut′s pod and testa.According to the results of sequencing,eight fragments were obtained finally.One was a known gene in peanut,the others were unknown genes.These seven sequences had been submitted to Genbank(Accession number DQ450065,DQ450066,DQ450067,DQ450068,DQ450069,DQ450070,DQ450071).And the method for isolating DD-PCR products using agarose gel electrophoresis was also discussed in this paper. |
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| Keywords: | peanut pod and testa specifically expressed gene DDRT-PCR |
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