| 牛羊胆汁中甘氨胆酸提取纯化工艺研究 |
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| 引用本文： | 赵珍,李培锋,李慧峰,关红,李玉娟.牛羊胆汁中甘氨胆酸提取纯化工艺研究[J].内蒙古农业大学学报(自然科学版),2006,27(2):9-12. |
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| 作者姓名： | 赵珍 李培锋 李慧峰 关红 李玉娟 |
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| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018
2. 包头农牧学校,包头,014300 |
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| 摘 要： | 采用盐析、萃取、柱层析等生物化学方法,从牛、羊胆汁中提取出了有效成分甘氨胆酸,并采用薄层层析法及薄层扫描法进行定量、定性检测,测得甘氨胆酸的纯度可达98%以上,牛羊胆汁甘氨胆酸的回收率分别为64%和62%。
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| 关 键 词： | 牛胆汁 甘氨胆酸 提取 分离纯化 |
| 文章编号： | 1009-3575（2006）02-0009-04 |
| 收稿时间： | 2006-03-03 |
| 修稿时间： | 2006-03-03 |
STUDIES ON EXTRACTION AND PURIFICATION TECHNIQUES OF GLYCOCHOLIC ACID FROM BOVINE AND SHEEP BILE |
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| ZHAO zhen,LI Pei-feng,LI Hui-feng,GUAN hong,LI Yu-juan.STUDIES ON EXTRACTION AND PURIFICATION TECHNIQUES OF GLYCOCHOLIC ACID FROM BOVINE AND SHEEP BILE[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2006,27(2):9-12. |
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| Authors: | ZHAO zhen LI Pei-feng LI Hui-feng GUAN hong LI Yu-juan |
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| Institution: | 1. Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot 010018 ; 2. Baotou Agricultural School,Baotou 014300,China |
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| Abstract: | Glycocholic Acid(GCA) was extracted and purified from Bovine and sheep bile by using the biochemistry techniques such as salting-out,extraction,dehydration,silica gel separation,and the qualitative and quantitative analysis of GCA were performed by using thin-layer chromatography.The purity of GCA was reached to 98%,and reclaim rate was 64% and 62%. |
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| Keywords: | Bovine bile glycocholic acid extraction separation and purification |
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