| 生石花植株离体再生及组培快繁研究 |
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| 引用本文： | 牟豪杰,王燕,吕永平,汪一婷,李海营.生石花植株离体再生及组培快繁研究[J].安徽农业科学,2016,44(33). |
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| 作者姓名： | 牟豪杰 王燕 吕永平 汪一婷 李海营 |
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| 作者单位： | 浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021;浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021 |
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| 基金项目： | 引进国际先进农业科学技术计划(948)项目(2011－G31)；浙江省农业科学院青年人才培养项目(2015R21R08E08)。 |
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| 摘 要： | 目的]建立生石花植株离体再生及组培快繁体系。方法]以生石花成熟种子为外植体,研究生石花组培快繁技术。结果]萌发后的幼苗在MS+0.50 mg/L 6-BA+0.05 mg/L NAA培养基上被成功诱导出愈伤组织;不添加6-BA的MS培养基有利于愈伤组织的分化,不定芽诱导率为4.65;在MS+0.05 mg/L 6-BA+0.01 mg/L NAA培养基上不定芽增殖率较高,可达5.60。结论]建立了生石花植株离体再生及快繁体系,有利于生石花种质资源保护和工厂化生产。
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| 关 键 词： | 生石花 愈伤组织 离体再生 增殖 生根 |
Plantlet Regeneration in vitro and Rapid Propagation of Lithops sp. |
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| Abstract: | Objective] The aim was to establish plantlet regeneration in vitro and rapid propagation of Lithops sp.Method] With mature seeds as explant, rapid propagation technique of Lithops sp.was studied. Result] Calli were induced from the young seedling on MS medium +0.50 mg/L 6-BA+0.05 mg/L NAA in this study.The results showed that the basic MS medium without 6-BA benefited the callus differentiation of Lithops sp., and the induction ratio of adventitious bud was 4.65;MS+0.05 mg/L 6-BA+0.01 mg/L NAA was the optimal medium for the multiplication of adventitious bud, and the multiplication ratio was 5.60.Conclusion] The plantlet regeneration and rapid propagation system of Lithops sp.were established, which has important guiding significance for the germplasm protection and industrial production of Lithops sp.. |
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| Keywords: | Lithops sp Callus Regeneration in vitro Proliferation Rooting |
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