The determination of dark adaptation time using electroretinography in conscious Miniature Schnauzer dogs |
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Authors: | Hyung-Ah Yu Man-Bok Jeong Shin-Ae Park Won-Tae Kim Se-Eun Kim Je-Min Chae Na-Young Yi Kang-Moon Seo |
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Affiliation: | Department of Veterinary Surgery and Ophthalmology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. |
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Abstract: | The optimal dark adaptation time of electroretinograms (ERG''s) performed on conscious dogs were determined using a commercially available ERG unit with a contact lens electrode and a built-in light source (LED-electrode). The ERG recordings were performed on nine healthy Miniature Schnauzer dogs. The bilateral ERG''s at seven different dark adaptation times at an intensity of 2.5 cd·s/m2 was performed. Signal averaging (4 flashes of light stimuli) was adopted to reduce electrophysiologic noise. As the dark adaptation time increased, a significant increase in the mean a-wave amplitudes was observed in comparison to base-line levels up to 10 min (p < 0.05). Thereafter, no significant differences in amplitude occured over the dark adaptation time. Moreover, at this time the mean amplitude was 60.30 ± 18.47 µV. However, no significant changes were observed for the implicit times of the a-wave. The implicit times and amplitude of the b-wave increased significantly up to 20 min of dark adaptation (p < 0.05). Beyond this time, the mean b-wave amplitudes was 132.92 ± 17.79 µV. The results of the present study demonstrate that, the optimal dark adaptation time when performing ERG''s, should be at least 20 min in conscious Miniature Schnauzer dogs. |
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Keywords: | dark adaptation time electroretinography Miniature Schnauzer dogs |
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