Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus |
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| Authors: | Amr E El Hakim Yasser E Shahein Amira M K Abouelella Mohamed E Selim |
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| Institution: | 1Molecular Biology Department, National Research Centre, Cairo, Egypt.;2Radiation Biology Department, NCRRT, Cairo, Egypt.;3Animal Health Department, Desert Research Centre, Mataria, Cairo, Egypt. |
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| Abstract: | The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between 6.8 and 7.2. Antibodies against the two GLPs, labeled I and II, were detected in the anti-whole tick, -whole larval, and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation. |
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| Keywords: | Boophilus annulatus glycoproteins larvae vaccination |
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