诺氟沙星在鲫体内的药动学及残留研究

研究了单剂量肌肉注射和多剂量混饲口灌给药方式下,诺氟沙星在鲫(Carassius auratus)体内的药物动力学和残留情况。结果显示:在(25.4±0.3)℃水温条件下,以每千克鱼体重10 mg的剂量单次给鲫肌肉注射诺氟沙星后,药物几乎在瞬间吸收,0.0333 h时血液中达到6.6708μg/mL,其血药浓度-时间数据用一级吸收二室模型描述较为合适,主要的药动学参数为:t1/2α、t1/2β、AUC和C l(s)分别为:0.4231 h、9.1613 h、17.8619μg.h/mL和0.5727 L/(kg.h)。在(23±1)℃水温条件下,以每千克鱼体重10 mg的剂量多次连续5 d混饲后,鲫停药后第8天肌肉、血清和肝脏中未检测到药物,此时肾脏中药物浓度已降到(0.0463±0.0134)μg/g,低于0.05μg/g。建议在(23±1)℃水温条件下,按每千克体重10 mg的剂量连续5 d混饲给鲫诺氟沙星,休药期至少为最后一次给药后的8 d。

The Pharmacokinetics and Residues of Norfloxacin in Carassius auratus

The present study investigated pharmacokinetics and residues of norfloxacin(NFX)in Carassius auratus.The drug concentrations in serum,muscle,liver and kidney were determined by Re-high performance liquid chromatography(Re-HPLC).The results showed that after a single-dosing intramuscular injection of 10 mg NFX/kg body weight in C.auratus at the water temperature of(25.4±0.3)℃,the drug concentration in the serum amounted to the largest(6.6708 μg/mL)immediately at the first sampling point at 0.033 h,the drug concentration-data in the serum can be described as a two-compartmental open model,the main pharmacokinetics parameters are t1/2α,t1/2β,AUC and Cl(s),which are 0.4231 h,9.1613 h,17.8619 μg·h/mL and 0.5727 L/(kg·h)respectively.And no drugs were detected in muscle,serum and liver in C.auratus,the concentration in kidney is(0.0463±0.0134)μg/g and lower than 0.05 μg/g on Day 8.Therefore,we suggest a withdrawal period should last at least for 8 days in C.auratus at the water temperature of(23±1)℃.