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| 苹果叶片蛋白质双向电泳样品制备方法的比较 | |
| 引用本文： | 曾广娟,李春敏,张新忠,滕云龙,董文轩.苹果叶片蛋白质双向电泳样品制备方法的比较[J].中国农学通报,2008,24(8):105-109. |
| 作者姓名： | 曾广娟 李春敏 张新忠 滕云龙 董文轩 |
| 作者单位： | 1. 沈阳农业大学园艺学院,沈阳110161;河北省农林科学院昌黎果树研究所,昌黎066600 2. 河北省农林科学院昌黎果树研究所,昌黎,066600 3. 中国农业大学园艺植物研究所,北京,100193 4. 沈阳农业大学园艺学院,沈阳,110161 |
| 基金项目： | 河北省自然科学基金资助项目 |
| 摘 要： | 为探索适用于苹果叶片蛋白质组学研究的样品制备方法，比较了三氯乙酸/丙酮沉淀法（TCA）、酚-甲醇/醋酸铵沉淀法和Tris-HCl提取法等3种总蛋白质的提取方法。制备的样品经双向电泳分离采用银染显色。结果3种方法分别得到450、374和1032个蛋白质点。认为Tris-HCl提取法为最适方法，所得图谱背景清晰，蛋白质信息量最大。 |
| 关 键 词： | 农民创业者 农民创业者 社会网络 创业资源 |
| 收稿时间： | 2008-05-04 |
| 修稿时间： | 2008-05-26 |
Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf |
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| Zeng Guangjuan,Li Chunmin,Zhang Xinzhong,Teng Yunlong,Dong Wenxuan.Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf[J].Chinese Agricultural Science Bulletin,2008,24(8):105-109. | |
| Authors: | Zeng Guangjuan Li Chunmin Zhang Xinzhong Teng Yunlong Dong Wenxuan |
| Institution: | College of Horticulture, Shenyang Agricultural Univercity, Shenyang 110161; Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Science, Changli 066600; Institute for Horticultural Plants, China Agricultural University, Beijing 100193 |
| Abstract: | In order to develop an efficient protein extraction method suitable for apple proteome analysis, three protocols for total protein extraction in apple leaf, trichloroacetic acid/acetone precipitation (TCA), phenol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared. The extracts were separated by two-dimensional electrophoresis (2-DE) followed by silver staining. The results showed that 450, 374 and 1032 protein spots were detected respectively on 2-DE gels. The Tris-HCl extraction was the most appropriate for apple leaf proteome analysis on account of the highest resolution and more informative 2-DE gels. |
| Keywords: | applezz proteomezz two-dimensional electrophoresis（2-DE）zz |
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