| 一种监测棉铃虫对Bt杀虫晶体蛋白抗性的技术 |
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| 引用本文： | 范贤林,芮昌辉,孟香清,梁桂梅,李永平.一种监测棉铃虫对Bt杀虫晶体蛋白抗性的技术[J].植物保护学报,2002,29(3):254-258. |
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| 作者姓名： | 范贤林 芮昌辉 孟香清 梁桂梅 李永平 |
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| 作者单位： | 1. 中国农业科学院植保所,北京,100094
2. 农业部全国农业技术推广中心,北京,100026 |
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| 基金项目： | 国家“863”计划资助项目(Z17-02-02) |
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| 摘 要： | 研究了以诊断试剂盒监测棉铃虫对苏云金杆菌(Bt)杀虫晶体蛋白(ICP)抗性的技术。将1日龄幼虫在含诊断剂量的饲料中(每毫升饲料含Bt ICP0.01mg)取食7天,以生长至3龄以上的个体作为抗性个体的判断指标;筛选出人工饲料中防腐剂剂量的最佳值为每350毫升饲料中含丙酸2.5ml。经对多个棉铃虫不同抗性种群的验证试验,证明诊断试剂盒检测抗性个体频率较为精确且应用简便。
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| 关 键 词： | 棉铃虫 Bt杀虫晶体蛋白 抗性监测 抗性诊断试剂盒 |
A diagnostic-dose detecting kit for monitoring cotton bollworm, H.armigera resistance to Bt ICP |
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| Fan Xianlin,Rui Changhui,Meng Xiangqing,Liang Guimei and Li Yongping.A diagnostic-dose detecting kit for monitoring cotton bollworm, H.armigera resistance to Bt ICP[J].Acta Phytophylacica Sinica,2002,29(3):254-258. |
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| Authors: | Fan Xianlin Rui Changhui Meng Xiangqing Liang Guimei and Li Yongping |
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| Institution: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094,National Agro-Technical Extension and Service Centre, Beijing 100026 and National Agro-Technical Extension and Service Centre, Beijing 100026 |
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| Abstract: | A diagnostic-dose detection kit was developed for monitoring cotton bollworm (CBW), H. armigera resistance to Bt ICP. The criteria for estimating a resistant individual is that a 24h-old larva can grow to 3rd instar after 7 days fed on artificial diet with the diagnostic dose of Bt ICP at 0.01 mg per1ml. The kit has good antiseptic effect with propionic acid at 2.5ml per 350ml artificial diet. It has been proved to be practical for detecting dynamic changes of resistant individual frequencies to Bt ICP in CBW population in the fields. |
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| Keywords: | Helicoverpa armigera Bt ICP resistance monitoring diagnostic dose |
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